iSouthern: Old-School Simplicity for Complex DNA Marks

We introduce immuno-Southern (iSouthern) – a cost-effective lab protocol.

By integrating restriction enzyme digestion with antibody detection, iSouthern delivers a dual readout that increases confidence, particularly when evaluating debated DNA modifications such as N6-methyldeoxyadenosine (6mA) in metazoan genomes.

Screen first. Sequence later - or independently validate your nanopore / bisulfite sequencing data by iSouthern.